Summary: Typewriter Therapeutics, Inc., Japan (Typewriter TX Japan GK) repurposes a class of genetic elements from various natural genomes and aims to develop an all-RNA-mediated gene writing platform via nonviral delivery to address unmet therapeutic needs. We are seeking a dedicated scientist to join our growing team and work on this new frontier technology in genome engineering. Reporting to the Sr./Principal Scientist, this scientist will join a collaborative and fun team. She/he is expected to be skilled in molecular and cell biology techniques and contribute to a rigorous scientific tone for our group. Prior experience in primary human cells (such as primary human hepatocytes) is desired. The successful applicant will have high growth potential in a fast-paced startup environment and make crucial contributions to new genome engineering tool development and next-generation human therapeutics. Key Responsibilities- Execute in vitro studies relevant to biological laboratory work (tissue culture, FACS, ELISA, activity assays, in vitro transcription (IVT), PCR and other molecular analytics) for internal programs- Play a central role in generating key data for evaluating gene writing efficiency and mechanism of action for in vitro studies- Design specific constructs, execute assays, and identify paths for constant optimization for higher efficacy in primary human cells- Make detailed observations, analyze, and interpret data- Present routinely to diverse audiences including internal research team and other internal/external stakeholders- Be a highly collaborative and energetic team player and a self-directed learner with a keen interest in focused, fast-paced research Qualifications- BS 8+ or MS 5+ or PhD 1+ years of experience in biological sciences or relevant life sciences-related field- Molecular biology techniques (e.g., nucleic acid extraction, PCR, in vitro transcription)- Cell biology techniques including cell culturing, transfection, transduction, and flow cytometry- Attention to detail, critical analysis of data, and troubleshooting ability- Strong written and oral communication skills; ability to work effectively as part of a team- Flexible and willing to work across multiple projects; maintain accurate lab notebooks; maintain equipment and a safe, organized bench Preferred Qualifications- Experience in gene editing or gene therapy (e.g., CRISPR, PRIME editing)- Experience culturing various primary cells (hepatocytes a plus)- Broad experience with cell-based functional assays (e.g., transfection, cytotoxicity, cytokine release, proliferation)- Proficiency in genomic profiling (RNA/DNA extraction, qPCR, dPCR, NGS), protein detection (ELISA, MSD, western blot), imaging and flow cytometry analysis- Experience in RNA production/purification; LNP or other nonviral delivery methods a plus; knowledge of key disease models a plus Working Conditions- Type: Permanent employment; Salary: ¥8,000,000–¥10,000,000 per year; Bonus: None- Location: Initial location Kashiwa-no-ha Office; possible relocation within Kanto in the future- Probation: 3 months; Hours: Discretionary labor system (deemed 8h; typically 9:00–18:00)- Holidays: Weekends/national holidays; special paid leave during Obon/New Year; paid leave 10 days after 6 months- Overtime: None (20h/month allowance included); Social insurance provided; Smoking prohibited Contact: Typewriter TX Japan GK, 5-4-6-613, Kashiwanoha, Kashiwa, Chiba, 277-0882, Japan; recruitjp@typewritertx.com