Overview: Typewriter TX Japan GK is a biotech startup originating from the University of Tokyo, developing next-generation gene editing technology (Gene Writing) for precise insertion of therapeutic genes. Based on cutting-edge transposon-based technologies, we aim to deliver innovative gene therapies for severe genetic disorders lacking effective treatments. We seek a highly motivated Research Technician/Research Associate to contribute to Gene Writing technologies. This role reports to our pharmacology scientist, in a collaborative and dynamic team. The ideal candidate has hands-on molecular and cellular biology experience, can independently execute protocol-based experiments, and generate high-quality, reproducible data. Responsibilities- Conduct in vitro experiments such as IVT, PCR, cell culture, FACS, ELISA, and bioactivity assays under guidance of pharmacology scientists, ensuring accuracy and efficiency- Generate critical data to evaluate gene editing efficiency and mechanisms of action in vitro- Perform detailed observations and produce high-quality, reproducible results- Maintain accurate/timely records; manage electronic data per company policies- Support lab operations (reagent procurement, vendor communication, inventory management, sample organization)- Contribute as a proactive and collaborative team player in a fast-paced environment Required Qualifications- Bachelor’s with 5+ years or Master’s with 3+ years of research experience in industry/academia, or recent PhD in biological sciences or related field- Proficiency in molecular biology (nucleic acid extraction, PCR, in vitro transcription)- Proficiency in cell biology (cell culture, transfection, transduction, flow cytometry)- Ability to follow protocols precisely and generate high-quality data; strong communication and teamwork skills- Flexibility to handle multiple protocols; accurate record keeping; ability to manage equipment/supplies/samples under direction; commitment to a safe, organized lab Preferred Qualifications (Not Required)- Experience with gene editing/gene therapy (CRISPR, PRIME editing)- Experience culturing primary cells (especially hepatocytes)- Experience with cell-based functional assays (cytotoxicity, cytokine release, proliferation)- Familiarity with genome profiling (qPCR, dPCR, NGS), protein detection (ELISA, MSD, Western blot), imaging, and flow cytometry analysis- Experience in RNA synthesis and purification; English communication skills a plus; strong attention to detail and troubleshooting Employment Conditions- Type: Permanent; Salary: ¥5,000,000–¥8,000,000 annually; Bonus: None- Location: Kashiwa-no-ha office (potential relocation within Kanto)- Probation: 3 months; Hours: Discretionary labor system (deemed 8h; typically 9:00–18:00)- Holidays: Weekends/national holidays; special paid leave during summer and New Year; 10 days paid leave after 6 months; substitute leave available- Overtime: None (20h/month allowance included); Social insurance provided; Smoking prohibited Contact: Typewriter TX Japan GK, 5-4-6-613, Kashiwanoha, Kashiwa, Chiba 277-0882, Japan; recruitjp@typewritertx.com